Fig. 6

In vivo biodistribution of free Cy5, Cy5-labeled CA-Oxi-αCD NPs and FA-CA-Oxi-αCD NPs in BGC-823 tumor-bearing mice. A In vivo images of mice 2, 4, 8, 12, 24 and 48 h after injection. The tumor area is within the red circle. B Ex vivo fluorescence image of the excised tumors and major tissues at 24 and 48 h post-injection. C Semiquantitative analysis of the fluorescence intensity of the tumor site 2, 4, 8, 12, 24 and 48 h after injection. D Semiquantitative analysis of fluorescence intensity in excised tumors at 24 and 48 h. E CLSM images of frozen tumor sections from mice after different Cy5-labeled NPs (red) treatments. Cell nuclei were stained with DAPI (blue). The scale bar represents 100 μm. F Semiquantitative analysis of CLSM images of tumor tissues from mice subjected to different treatments at 24 and 48 h. (i) Free Cy5, (ii) Cy5-labeled CA-Oxi-αCD NPs, (iii) Cy5-labeled FA-CA-Oxi-αCD NPs. *, significantly different at p < 0.05; **, significantly different at p < 0.01; ***, significantly different at p < 0.001