Table 1 Oligonucleotide sequences of apoptotic genes used for expression studies by RT-PCR in response to CW-AgNPs treatment
Sr. no | Gene | Oligonucleotide sequence | Tm (ºC) | Amplicon size (bp) |
|---|---|---|---|---|
1 | P53 | FP 5ʹ-ACTAAGCGAGCACTGCCCAA-3ʹ RP 5ʹ-ATGGCGGGAGGTAGACTGAC-3ʹ | 54 56 | 175 |
2 | Caspase-3 | FP 5ʹ-GTG GCA TTG AGA CAG ACA GTG G-3ʹ RP 5ʹ-GCCAAG AAT AAT AAC CAG GTG C-3ʹ | 57 54 | 110 |
3 | Bcl2 | FP 5ʹ-TGTGGCCTTCTTTGAGTTCG-3ʹ RP 5ʹ-TCACTTGTGGCCCAGATAGG-3ʹ | 52 54 | 150 |
4 | Actin | FP 5ʹ-TCT GGC ACC ACA CCT TCT ACA ATG-3ʹ RP 5ʹ-AGC ACA GCC TGG ATA GCA ACG-3ʹ | 57 56 | 200 |