Fig. 9
A Representative histogram showing relative levels of p53, caspase-3, Bcl2 proteins from MCF-7 cells resolved in western blot were quantitated by densitometric analysis. Error bars indicate the standard error of the mean (SE) for three independent experiments (n = 3). Significant differences assessed by Student’s t test are indicated by (***p < 0.005) and are found to be increased significantly in samples obtained from 24-h and 48-h post-exposure periods as compared to control. B Representative western blot showing expression of p53, caspase-3 and Bcl2 protein from MCF-7 cells exposed to IC50 concentration (8.48 µg/ml) of CW-AgNPs with different time points (24 h and 48 h) (N = 3). Lane 1 is control; lanes 2 and 3 are cell protein extracts from CW-AgNPs exposed samples at 24-h and 48-h exposure time. C Representative histogram showing relative levels of p53, caspase-3, and Bcl2 cDNA of MCF-7 cells. Significant differences in levels of p53 and caspase-3 are indicated by (***p < 0.005) and found to be increased significantly in samples obtained from 24-h and 48-h post-exposure periods as compared to control. D Representative amplicons of p53, caspase-3, Bcl2 and Actin genes from MCF-7, cells exposed to IC50 concentration (8.48 µg/ml) of CW-AgNPs. Lane 1 is control; lanes 2 and 3 are mRNA levels from CW-AgNPs exposed samples at 24-h and 48-h exposure time