Fig. 8

A Confirmation of apoptosis by Annexin-V-FITC/PI staining assay. a Control MCF-7 cells. b MCF-7 cells treated with IC50: 8.48 µg/ml CW-AgNPs for 24 h. The cells were stained with Annexin-V-FITC and propidium iodide and the percentage of apoptotic cell population was analyzed by flow cytometry. B Cell cycle arrest assay where a control cells and b MCF-7 cells were treated with 8.48 µg/ml CW-AgNPs for 24 h and the cell cycle distribution was analyzed by flow cytometry