Fig. 3

Cytotoxicity test on monolayer cultures of HT-29 CRC malignant cells and CCD-18Co fibroblast-like, healthy colorectal cells. Cells were treated with different concentrations of L-ZnO and YSA-L-ZnO (15, 3, 50, 75 and 100 µg/mL). Cell viability was measured trough WST-1 metabolic assay after cells exposure to the nanoparticles. Data are expressed as normalized percentage with respect to the control sample, which was considered 100% viable. *p < 0.0332, **p < 0.0021, ***p < 0.0002, ****p < 0.0001