Fig. 4

Cytotoxic effects of synthesized samples, including P, P-P, P-P-Cur, and Cur, on A549 and HGF cells after 24 h, 48 h, and 72 h of treatment with 50 nM, 100 nM, 150 nM, and 200 nM concentrations. Based on data, around 86 and 93% of cell viabilities are observed for A549 cells after 24 of treatment with 50 nM and 200 nM concentrations of P samples which presents the biocompatibility and biodegradability of biopolymer. Regarding P-P nano-beads, about 97% cell viability is seen at 200 nM concentration at 24 h of post-treatment while cell viabilities are remarkably declined to 63% and 41% for concentrations of 50 nM and 200 nM after 24 h of treatment, respectively. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001