Fig. 2

Intracellular uptake and localisation of AuNPs within FaDu, CAL27 and CAL33 cells under atmospheric oxygen conditions using Cytoviva darkfield/hyperspectral imaging. Cells were treated with AuNPs for 24 h, fixed and mounted with DAPI before imaging. Representative images include enhanced dark field and the corresponding hyperspectral images, overlaid with a spectral angle map (SAM) representing AuNPs in red