Fig. 4
From: Functional targeted therapy for glioma based on platelet membrane-coated nanogels
Evaluation of BBB penetration efficiency by in vitro and in vivo models. A In vitro blood–brain barrier model. B Flow cytometry analysis of the fluorescence intensity of C6 cells in the in vitro BBB model. C Confocal microscope map of C6 cells incubated with DOX, DOX@NGs, or DOX@PNGs with same DOX concentration of 5 μg/mL for 24 h; scale bar = 20 μm. D IVIS image of the mouse brain 24 h after free Cy5, Cy5-NGs, or Cy5-PNGs injection via the tail vein. E Fluorescence semi-quantitative analysis of C. F Flow cytometry analysis of the relative fluorescence intensity of C6 cells in the in vivo BBB model. The error bars represent the means ± SD; n = 3; *p < 0.05, **p < 0.01, ***p < 0.001